Recombinant phycobiliproteins with enhanced fluorescence and photochemical properties

Donald A. Bryant, Wendy M. Schluchter, Richard M. Alvey, Avijit Biswas

Research output: Other contribution

Abstract

Novel fluorescent phycobiliprotein fusion proteins and methods of use are described. The novel phycobiliproteins can be produced in a cell comprising two or more heterodimeric lyases, an apoprotein and a bilin reductase, which components react to form the phycobiliprotein fusion protein. Also described are phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous, fluorescent, phycobiliprotein domain even in anoxic conditions.
Original languageAmerican English
StatePublished - Sep 8 2011
Externally publishedYes

Disciplines

  • Biochemistry
  • Biology
  • Molecular Biology

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