SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis.

Loralyn M. Cozy; Andrew M. Phillips; Rebecca A. Calvo; Ashley R. Bate; Yi Huang Hsueh; Richard Bonneau; Patrick Eichenberger; Daniel B. Kearns

doi:10.1111/j.1365-2958.2012.08003.x

SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis.

Loralyn M. Cozy, Andrew M. Phillips, Rebecca A. Calvo, Ashley R. Bate, Yi Huang Hsueh, Richard Bonneau, Patrick Eichenberger, Daniel B. Kearns

Research output: Journal Article › Article › peer-review

Abstract

<div class="line" id="line-15"> Exponentially growing Bacillus subtilis cultures are epigenetically differentiated into two subpopulations in which cells are either ON or OFF for σd&hyphen;dependent gene expression: a pattern suggestive of bistability. The gene encoding σD, sigD , is part of the 31&hyphen;gene fla/che operon where its location at the 3′ end, 25&emsp;kb away from the strong Pfla/che promoter, determines its expression level relative to a threshold. Here we show that addition of a single extra copy of the slrA gene in the chromosome inhibited σd&hyphen;dependent gene expression. SlrA together with SinR and SlrR reduced sigD transcript by potentiating a distance&hyphen;dependent decrease in fla/che operon transcript abundance that was not mediated by changes in expression from the Pfla/che promoter. Consistent with acting upstream of σD, SlrA/SinR/SlrR was bypassed by artificial ectopic expression of sigD and hysteretically maintained for 20 generations by engaging the sigD gene at the native locus. SlrA/SinR/SlrR was also bypassed by increasing fla/che transcription and resulted in a hypersensitive output in flagellin expression. Thus, flagellin gene expression demonstrated hypersensitivity and hysteresis and we conclude that σd&hyphen;dependent gene expression is bistable.</div>

Original language	American English
Journal	Molecular Microbiology
Volume	83
DOIs	https://doi.org/10.1111/j.1365-2958.2012.08003.x
State	Published - Jan 3 2012
Externally published	Yes

Disciplines

Genetics
Biology
Molecular Biology

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@article{2addeadecec54d86bad4676e9f163cfd,

title = "SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis.",

abstract = " Exponentially growing Bacillus subtilis cultures are epigenetically differentiated into two subpopulations in which cells are either ON or OFF for σd&hyphen;dependent gene expression: a pattern suggestive of bistability. The gene encoding σD, sigD , is part of the 31&hyphen;gene fla/che operon where its location at the 3′ end, 25&emsp;kb away from the strong Pfla/che promoter, determines its expression level relative to a threshold. Here we show that addition of a single extra copy of the slrA gene in the chromosome inhibited σd&hyphen;dependent gene expression. SlrA together with SinR and SlrR reduced sigD transcript by potentiating a distance&hyphen;dependent decrease in fla/che operon transcript abundance that was not mediated by changes in expression from the Pfla/che promoter. Consistent with acting upstream of σD, SlrA/SinR/SlrR was bypassed by artificial ectopic expression of sigD and hysteretically maintained for 20 generations by engaging the sigD gene at the native locus. SlrA/SinR/SlrR was also bypassed by increasing fla/che transcription and resulted in a hypersensitive output in flagellin expression. Thus, flagellin gene expression demonstrated hypersensitivity and hysteresis and we conclude that σd&hyphen;dependent gene expression is bistable.",

author = "Cozy, {Loralyn M.} and Phillips, {Andrew M.} and Calvo, {Rebecca A.} and Bate, {Ashley R.} and Hsueh, {Yi Huang} and Richard Bonneau and Patrick Eichenberger and Kearns, {Daniel B.}",

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year = "2012",

month = jan,

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doi = "10.1111/j.1365-2958.2012.08003.x",

language = "American English",

volume = "83",

journal = "Molecular Microbiology",

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T1 - SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis.

AU - Cozy, Loralyn M.

AU - Phillips, Andrew M.

AU - Calvo, Rebecca A.

AU - Bate, Ashley R.

AU - Hsueh, Yi Huang

AU - Bonneau, Richard

AU - Eichenberger, Patrick

AU - Kearns, Daniel B.

N1 - Enter your email address below. If your address has been previously registered, you will receive an email with instructions on how to reset your password. If you don't receive an email, you should register as a new user

PY - 2012/1/3

Y1 - 2012/1/3

N2 - Exponentially growing Bacillus subtilis cultures are epigenetically differentiated into two subpopulations in which cells are either ON or OFF for σd&hyphen;dependent gene expression: a pattern suggestive of bistability. The gene encoding σD, sigD , is part of the 31&hyphen;gene fla/che operon where its location at the 3′ end, 25&emsp;kb away from the strong Pfla/che promoter, determines its expression level relative to a threshold. Here we show that addition of a single extra copy of the slrA gene in the chromosome inhibited σd&hyphen;dependent gene expression. SlrA together with SinR and SlrR reduced sigD transcript by potentiating a distance&hyphen;dependent decrease in fla/che operon transcript abundance that was not mediated by changes in expression from the Pfla/che promoter. Consistent with acting upstream of σD, SlrA/SinR/SlrR was bypassed by artificial ectopic expression of sigD and hysteretically maintained for 20 generations by engaging the sigD gene at the native locus. SlrA/SinR/SlrR was also bypassed by increasing fla/che transcription and resulted in a hypersensitive output in flagellin expression. Thus, flagellin gene expression demonstrated hypersensitivity and hysteresis and we conclude that σd&hyphen;dependent gene expression is bistable.

AB - Exponentially growing Bacillus subtilis cultures are epigenetically differentiated into two subpopulations in which cells are either ON or OFF for σd&hyphen;dependent gene expression: a pattern suggestive of bistability. The gene encoding σD, sigD , is part of the 31&hyphen;gene fla/che operon where its location at the 3′ end, 25&emsp;kb away from the strong Pfla/che promoter, determines its expression level relative to a threshold. Here we show that addition of a single extra copy of the slrA gene in the chromosome inhibited σd&hyphen;dependent gene expression. SlrA together with SinR and SlrR reduced sigD transcript by potentiating a distance&hyphen;dependent decrease in fla/che operon transcript abundance that was not mediated by changes in expression from the Pfla/che promoter. Consistent with acting upstream of σD, SlrA/SinR/SlrR was bypassed by artificial ectopic expression of sigD and hysteretically maintained for 20 generations by engaging the sigD gene at the native locus. SlrA/SinR/SlrR was also bypassed by increasing fla/che transcription and resulted in a hypersensitive output in flagellin expression. Thus, flagellin gene expression demonstrated hypersensitivity and hysteresis and we conclude that σd&hyphen;dependent gene expression is bistable.

UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2012.08003.x/abstract

U2 - 10.1111/j.1365-2958.2012.08003.x

DO - 10.1111/j.1365-2958.2012.08003.x

M3 - Article

VL - 83

JO - Molecular Microbiology

JF - Molecular Microbiology

ER -

SlrA/SinR/SlrR inhibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of σ(D) in Bacillus subtilis.

Abstract

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