The Isolation and Characterization of Novel Bacteriophages from Central Illinois

Anthony Bohner; Juie Anne Canter; Drew Cullet; Crystal Diaz; Kati Forman; Munia Mustafsa; Catlin Arrington; Elyse Borchik; Ethan Gelke; Ryan Holden; Sam Sorenson; Brenden Wall; Da Wang; Lauren Awdziejczyk; Stephen Whitfield; David Bollivar, Faculty Advisor; Loni Walker, Faculty Advisor; Abigail Kerr

The Isolation and Characterization of Novel Bacteriophages from Central Illinois

Anthony Bohner, Juie Anne Canter, Drew Cullet, Crystal Diaz, Kati Forman, Munia Mustafsa, Catlin Arrington, Elyse Borchik, Ethan Gelke, Ryan Holden, Sam Sorenson, Brenden Wall, Da Wang, Lauren Awdziejczyk, Stephen Whitfield, David Bollivar, Faculty Advisor, Loni Walker, Faculty Advisor, Abigail Kerr

Illinois Wesleyan University

Research output: Conference Poster › Presentation

Abstract

Members of the Illinois Wesleyan University General Biology Science Education Alliance (SEA) laboratory isolated and characterized a number of distinctive mycobacteriophages. Each student collected soil samples from the central Illinois area then used streak assays and titrations to isolate unique phages that infect Mycobacterium smegmatis . Transmission electron microscopy (TEM) and DNA restriction enzyme digests were used to determine the morphology and tentative cluster placement of each phage. By the end of the semester, fifteen novel phages were isolated with a wide range of characteristics, despite the small sampling area. This data was submitted to the Mycobacteriophage DataBase. After analysis and class discussion, the DNA from three different phages were sent to the University of Pittsburgh for genome sequencing.

Original language	American English
State	Published - Apr 14 2012

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title = "The Isolation and Characterization of Novel Bacteriophages from Central Illinois",

abstract = " Members of the Illinois Wesleyan University General Biology Science Education Alliance (SEA) laboratory isolated and characterized a number of distinctive mycobacteriophages. Each student collected soil samples from the central Illinois area then used streak assays and titrations to isolate unique phages that infect Mycobacterium smegmatis . Transmission electron microscopy (TEM) and DNA restriction enzyme digests were used to determine the morphology and tentative cluster placement of each phage. By the end of the semester, fifteen novel phages were isolated with a wide range of characteristics, despite the small sampling area. This data was submitted to the Mycobacteriophage DataBase. After analysis and class discussion, the DNA from three different phages were sent to the University of Pittsburgh for genome sequencing.",

author = "Anthony Bohner and Canter, {Juie Anne} and Drew Cullet and Crystal Diaz and Kati Forman and Munia Mustafsa and Catlin Arrington and Elyse Borchik and Ethan Gelke and Ryan Holden and Sam Sorenson and Brenden Wall and Da Wang and Lauren Awdziejczyk and Stephen Whitfield and {Bollivar, Faculty Advisor}, David and {Walker, Faculty Advisor}, Loni and Abigail Kerr",

year = "2012",

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day = "14",

language = "American English",

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TY - CONF

T1 - The Isolation and Characterization of Novel Bacteriophages from Central Illinois

AU - Bohner, Anthony

AU - Canter, Juie Anne

AU - Cullet, Drew

AU - Diaz, Crystal

AU - Forman, Kati

AU - Mustafsa, Munia

AU - Arrington, Catlin

AU - Borchik, Elyse

AU - Gelke, Ethan

AU - Holden, Ryan

AU - Sorenson, Sam

AU - Wall, Brenden

AU - Wang, Da

AU - Awdziejczyk, Lauren

AU - Whitfield, Stephen

AU - Bollivar, Faculty Advisor, David

AU - Walker, Faculty Advisor, Loni

AU - Kerr, Abigail

PY - 2012/4/14

Y1 - 2012/4/14

N2 - Members of the Illinois Wesleyan University General Biology Science Education Alliance (SEA) laboratory isolated and characterized a number of distinctive mycobacteriophages. Each student collected soil samples from the central Illinois area then used streak assays and titrations to isolate unique phages that infect Mycobacterium smegmatis . Transmission electron microscopy (TEM) and DNA restriction enzyme digests were used to determine the morphology and tentative cluster placement of each phage. By the end of the semester, fifteen novel phages were isolated with a wide range of characteristics, despite the small sampling area. This data was submitted to the Mycobacteriophage DataBase. After analysis and class discussion, the DNA from three different phages were sent to the University of Pittsburgh for genome sequencing.

AB - Members of the Illinois Wesleyan University General Biology Science Education Alliance (SEA) laboratory isolated and characterized a number of distinctive mycobacteriophages. Each student collected soil samples from the central Illinois area then used streak assays and titrations to isolate unique phages that infect Mycobacterium smegmatis . Transmission electron microscopy (TEM) and DNA restriction enzyme digests were used to determine the morphology and tentative cluster placement of each phage. By the end of the semester, fifteen novel phages were isolated with a wide range of characteristics, despite the small sampling area. This data was submitted to the Mycobacteriophage DataBase. After analysis and class discussion, the DNA from three different phages were sent to the University of Pittsburgh for genome sequencing.

UR - https://digitalcommons.iwu.edu/jwprc/2012/posters/3

M3 - Presentation

ER -

The Isolation and Characterization of Novel Bacteriophages from Central Illinois

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